Materials and Methods

Twenty-four items of information packages to be sterilized were 6 pieces of holding clamp cylinder, 6 pieces of changing bowl, 6 storage tanks and 6 sterile containers.

Each group takes 4 items out of 4 items, altogether 4 pieces, less than 10% of the sterilizer cabinet volume, is the test group, 5 pieces of the remaining 4 items, altogether 20 pieces, more than 10% of the sterilizer cabinet volume Above, the control group.

After the sterilizing cycle is pulsating vacuum three times, the temperature is increased to 132134e, the pressure is 0.21 mPa, and the sterilization is continued for 6 min. The steam is exhausted, dried and finished. For a sterilization cycle.

method

The 4 items in the test group and the 20 items in the control group were respectively placed in a sterilizer. After a sterilizing cycle, samples were sampled using the surface sampling method in a sterile room and the specimens were cultured in bacteria.

condition

Sterilized growth control group 205>101 Surface growth of 20 items aseptically 100 sampling method The above results show that the loading amount of the test group items is less than 10% of the sterilizer cabinet interior volume, and the sterilization pass rate is 25%, while that of the control group The filling volume is greater than 10% of the sterilizer's indoor volume, and the sterilization pass rate is 100%. V2 = 5.22, P Discussion

Small loading effect The conventional pre-vacuum sterilization method enables the vacuum degree to be pumped to 2.67 kPa (20 mmHg) absolute pressure. The amount of items in the cabinet can not be less than 10% of the volume of the cabinet. Otherwise, small quantity effects will easily occur and the effect will be eliminated. Bacteria effect, the phenomenon of poor sterilization effect of this kind of loaded items is the effect of small quantity. Therefore, in the article sterilization operation, we must pay attention to the effect of small quantities, and our test results also illustrate this problem.

The small loading effect results from the fact that after vacuuming in the pre-vacuum sterilizer, the remaining air can easily infiltrate into the interior of the article and wrap around the article to form an air barrier, which hinders heat conduction and leads to sterilization failure.

When the pulsating vacuum is more than 3 times, it can also achieve the effect of removing 98% of cold air once in a vacuum of 2.67kPa (20mmHg). The test results of the above two sets of tests show that when sterilizing, the loading amount of the articles is less than 10% of the indoor volume of the sterilizing cabinet, which seriously affects the sterilizing quality of the articles, and the loading amount is more than 10%, and the sterilizing qualification rate reaches 100%.

Therefore, in the daily sterilization work, 12 items cannot be urgently needed, neglecting the small-volume effect and affecting the sterilization quality. In addition, the sterilizers and nurses must intensify the professional theoretical study, and through business learning, make everyone realize the importance of the relationship between the amount of goods loaded and sterilization quality.

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